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lipid peroxidation assay kit  (MedChemExpress)


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    MedChemExpress lipid peroxidation assay kit
    Lipid Peroxidation Assay Kit, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mda+kit/Lipid+Peroxidation+(MDA)+Assay+Kit/pm42468217-231-8-12
    Average 94 stars, based on 16 article reviews
    lipid peroxidation assay kit - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Multiple Displacement Amplification:

    Article Title: Glycyrrhetinic acid ameliorates chronic heart failure via the Nrf2 pathway.
    Article Snippet: .. Glutathione (GSH) and malondialdehyde (MDA) levels were measured according to the manufacturers' protocols using the GSH colorimetric assay kit (EIAGSHC, Invitrogen, Carlsbad, CA, USA) and the MDA kit (HY-K0319, MedChemExpress), respectively. .. JC-1 staining for mitochondrial membrane potential Cells were collected, washed twice with PBS, and incubated with JC-1 dye (ab113850, Abcam) at 37 °C with 5% CO2 for 20 minutes.

    Article Title: circFADS2 inhibits ferroptosis associated with IGF2BP2 -dependent SLC7A11 m6A modification in colorectal cancer cells
    Article Snippet: Finally, the protein bands were developed using enhanced chemiluminescence (ECL) reagent (32106, Thermo Fisher Scientific) and analyzed for OD using ImageJ image analysis software with GAPDH as the internal reference. .. A reduced GSH Colorimetric Assay Kit (EIAGSHC, Thermo Fisher Scientific), MDA Kit (HY-K0319, MedChemExpress, Monmouth Junction, NJ, USA) and cell total iron colorimetric assay kit (EEA009, Thermo Fisher Scientific) was used to detect GSH, MDA, ROS, and Fe 2+ in cells. .. This study was approved by the Animal Committee of Affiliated Nanhua Hospital, University of South China (No. 20240302) and conducted in compliance with the national guidelines for the care and use of animals.

    GSH Assay:

    Article Title: Glycyrrhetinic acid ameliorates chronic heart failure via the Nrf2 pathway.
    Article Snippet: .. Glutathione (GSH) and malondialdehyde (MDA) levels were measured according to the manufacturers' protocols using the GSH colorimetric assay kit (EIAGSHC, Invitrogen, Carlsbad, CA, USA) and the MDA kit (HY-K0319, MedChemExpress), respectively. .. JC-1 staining for mitochondrial membrane potential Cells were collected, washed twice with PBS, and incubated with JC-1 dye (ab113850, Abcam) at 37 °C with 5% CO2 for 20 minutes.

    Article Title: circFADS2 inhibits ferroptosis associated with IGF2BP2 -dependent SLC7A11 m6A modification in colorectal cancer cells
    Article Snippet: Finally, the protein bands were developed using enhanced chemiluminescence (ECL) reagent (32106, Thermo Fisher Scientific) and analyzed for OD using ImageJ image analysis software with GAPDH as the internal reference. .. A reduced GSH Colorimetric Assay Kit (EIAGSHC, Thermo Fisher Scientific), MDA Kit (HY-K0319, MedChemExpress, Monmouth Junction, NJ, USA) and cell total iron colorimetric assay kit (EEA009, Thermo Fisher Scientific) was used to detect GSH, MDA, ROS, and Fe 2+ in cells. .. This study was approved by the Animal Committee of Affiliated Nanhua Hospital, University of South China (No. 20240302) and conducted in compliance with the national guidelines for the care and use of animals.

    Colorimetric Assay:

    Article Title: circFADS2 inhibits ferroptosis associated with IGF2BP2 -dependent SLC7A11 m6A modification in colorectal cancer cells
    Article Snippet: Finally, the protein bands were developed using enhanced chemiluminescence (ECL) reagent (32106, Thermo Fisher Scientific) and analyzed for OD using ImageJ image analysis software with GAPDH as the internal reference. .. A reduced GSH Colorimetric Assay Kit (EIAGSHC, Thermo Fisher Scientific), MDA Kit (HY-K0319, MedChemExpress, Monmouth Junction, NJ, USA) and cell total iron colorimetric assay kit (EEA009, Thermo Fisher Scientific) was used to detect GSH, MDA, ROS, and Fe 2+ in cells. .. This study was approved by the Animal Committee of Affiliated Nanhua Hospital, University of South China (No. 20240302) and conducted in compliance with the national guidelines for the care and use of animals.



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    D-EVs Counteract NPC Senescence by Suppressing Ferroptosis. (A) KEGG pathway analysis of DEGs in senescent NPCs following treatment with D-EVs or not. (B-C) GSEA plots showing significant enrichment of ferroptosis and cell cycle in senescent NPCs. (D-E) Heatmap quantification of key genes involved in ferroptosis and cell cycle. (F) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with different experimental conditions. (G) Representative images of C11-BODIPY 581/591 staining to detect lipid <t>peroxidation</t> (green) in the control, TBHP, Era, Era + Fer-1, or TBHP + Fer-1 groups. (H-I) Quantitative assessment of malondialdehyde <t>(MDA)</t> levels (H) and glutathione (GSH) levels (I) in the control, TBHP, N-EVs, D-EVs, or D-EVs + Era groups. (J) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with PBS, N-EVs, D-EVs, or D-EVs + Era. (K) Confocal analysis of GPX4 with IF staining in the control, TBHP, N-Evs, D-EVs, and D-EVs + Era group. (L) Flow cytometry analysis of cell cycle distribution in the above experimental conditions. Statistical comparisons were performed between the experimental group and the TBHP-induced group. The data were presented as mean ± SD. n = 3, ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
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    D-EVs Counteract NPC Senescence by Suppressing Ferroptosis. (A) KEGG pathway analysis of DEGs in senescent NPCs following treatment with D-EVs or not. (B-C) GSEA plots showing significant enrichment of ferroptosis and cell cycle in senescent NPCs. (D-E) Heatmap quantification of key genes involved in ferroptosis and cell cycle. (F) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with different experimental conditions. (G) Representative images of C11-BODIPY 581/591 staining to detect lipid <t>peroxidation</t> (green) in the control, TBHP, Era, Era + Fer-1, or TBHP + Fer-1 groups. (H-I) Quantitative assessment of malondialdehyde <t>(MDA)</t> levels (H) and glutathione (GSH) levels (I) in the control, TBHP, N-EVs, D-EVs, or D-EVs + Era groups. (J) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with PBS, N-EVs, D-EVs, or D-EVs + Era. (K) Confocal analysis of GPX4 with IF staining in the control, TBHP, N-Evs, D-EVs, and D-EVs + Era group. (L) Flow cytometry analysis of cell cycle distribution in the above experimental conditions. Statistical comparisons were performed between the experimental group and the TBHP-induced group. The data were presented as mean ± SD. n = 3, ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
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    Image Search Results


    D-EVs Counteract NPC Senescence by Suppressing Ferroptosis. (A) KEGG pathway analysis of DEGs in senescent NPCs following treatment with D-EVs or not. (B-C) GSEA plots showing significant enrichment of ferroptosis and cell cycle in senescent NPCs. (D-E) Heatmap quantification of key genes involved in ferroptosis and cell cycle. (F) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with different experimental conditions. (G) Representative images of C11-BODIPY 581/591 staining to detect lipid peroxidation (green) in the control, TBHP, Era, Era + Fer-1, or TBHP + Fer-1 groups. (H-I) Quantitative assessment of malondialdehyde (MDA) levels (H) and glutathione (GSH) levels (I) in the control, TBHP, N-EVs, D-EVs, or D-EVs + Era groups. (J) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with PBS, N-EVs, D-EVs, or D-EVs + Era. (K) Confocal analysis of GPX4 with IF staining in the control, TBHP, N-Evs, D-EVs, and D-EVs + Era group. (L) Flow cytometry analysis of cell cycle distribution in the above experimental conditions. Statistical comparisons were performed between the experimental group and the TBHP-induced group. The data were presented as mean ± SD. n = 3, ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Journal: Bioactive Materials

    Article Title: Microenvironment-educated MSC-EVs loaded injectable smart hydrogel for targeting senescent nucleus pulposus cells and inhibiting ferroptosis against intervertebral disc degeneration

    doi: 10.1016/j.bioactmat.2026.02.030

    Figure Lengend Snippet: D-EVs Counteract NPC Senescence by Suppressing Ferroptosis. (A) KEGG pathway analysis of DEGs in senescent NPCs following treatment with D-EVs or not. (B-C) GSEA plots showing significant enrichment of ferroptosis and cell cycle in senescent NPCs. (D-E) Heatmap quantification of key genes involved in ferroptosis and cell cycle. (F) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with different experimental conditions. (G) Representative images of C11-BODIPY 581/591 staining to detect lipid peroxidation (green) in the control, TBHP, Era, Era + Fer-1, or TBHP + Fer-1 groups. (H-I) Quantitative assessment of malondialdehyde (MDA) levels (H) and glutathione (GSH) levels (I) in the control, TBHP, N-EVs, D-EVs, or D-EVs + Era groups. (J) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with PBS, N-EVs, D-EVs, or D-EVs + Era. (K) Confocal analysis of GPX4 with IF staining in the control, TBHP, N-Evs, D-EVs, and D-EVs + Era group. (L) Flow cytometry analysis of cell cycle distribution in the above experimental conditions. Statistical comparisons were performed between the experimental group and the TBHP-induced group. The data were presented as mean ± SD. n = 3, ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Article Snippet: The level of lipid peroxidation was measured by Lipid Peroxidation MDA Assay Kit (Beyotime, China) according to the manufacturer's protocol.

    Techniques: Western Blot, Staining, Control, Flow Cytometry